⚠ For Research Purposes Only — Not for human or veterinary therapeutic use. Consult a licensed veterinarian before use.

Veterinary Peptide Protocol Library

Evidence-based dosing protocols organized by condition and species. Built by pharmaceutical scientists for informed veterinary professionals and researchers.

Research reference only. All protocols should be reviewed and supervised by a licensed veterinarian.
Joint, Tendon & Injury Recovery Protocols
Peptides with the strongest evidence base for soft-tissue healing, joint repair, and post-surgical recovery

BPC-157

Best Seller — Evidence Level B
Dog Cat Horse

The primary tissue repair peptide. Promotes angiogenesis, accelerates tendon/ligament healing, and protects GI mucosa. Most versatile peptide in veterinary practice.

SpeciesDoseRouteCycle
Dog2–5 mcg/kg once dailySC injection4–8 weeks
Cat100–200 mcg once dailySC injection4–6 weeks
Horse0.5–2 mcg/kg once dailySC or IA6–8 weeks

Indications: Tendon tears, ligament injuries, GI ulcers/IBD, wound healing, post-surgical recovery, joint inflammation

⚠ Contraindicated in active malignancy

TB-500 (Thymosin Beta-4)

Equine Favorite — Evidence Level A
Dog Horse

The gold standard in equine recovery. Promotes systemic muscle fiber repair, reduces inflammation, and accelerates tendon remodeling. Trusted by elite equine programs.

SpeciesLoading (Wk 1–4)Maintenance
Dog2.5 mg SC 2×/wk2.5 mg SC weekly
Horse10–20 mg SC 2×/wk10 mg SC weekly

Indications: Muscle tears, tendon injuries, soft-tissue damage, performance maintenance

⚠ Avoid in pregnancy

GHK-Cu

Wound & Coat — Evidence Level B
Dog Cat Horse

Copper tripeptide naturally found in plasma. Stimulates collagen I & III synthesis, accelerates wound closure, improves coat quality. Available topical or injectable.

RouteDogCatHorse
Topical0.1–0.5% cream 1–2×/day0.05–0.1% 1×/day0.1–0.3% gel 1–2×/day
SC injection0.5–1 mg 3×/wkNot preferred5–10 mg 3×/wk

Indications: Wound healing, post-surgical sites, coat/skin health, connective tissue support

AOD 9604

Fat + Joint — Evidence Level B
Dog

Modified GH fragment with dual action: lipolytic AND cartilage-protective. Ideal for overweight dogs with concurrent arthritis. No blood sugar effects.

SpeciesDoseTimingCycle
Dog300–500 mcg SC dailyBefore exercise12–16 weeks

Indications: Obesity with arthritis, cartilage repair, fat metabolism support

Immune Support & Chronic Illness Protocols
Peptides for viral infections, chronic disease, immune deficiency, and liver/detox support

Thymosin Alpha-1

FeLV/FIV Support — Evidence Level A
Dog Cat Horse

The immune system master regulator. Activates NK cells, dendritic cells, and T-cell maturation. Used clinically for 30+ years in human medicine for hepatitis and HIV immune support.

Species / ConditionDoseFrequencyDuration
Dog — chronic infection900 mcg/m² SC2×/week4–6 weeks
Dog — cancer adjunct900 mcg/m² SC2–3×/weekOngoing
Cat — FeLV/FIV active500 mcg SC2×/week6–8 weeks
Cat — FeLV/FIV maintenance500 mcg SCWeeklyOngoing
Horse2–4 mg SC2×/week4 weeks
BSA Reference (Dog): 5kg=0.29m² | 10kg=0.46m² | 20kg=0.74m² | 30kg=0.96m² | 40kg=1.17m²

⚠ Use with caution in autoimmune disease; avoid in transplant patients on immunosuppression

KPV

Anti-Inflammatory — Evidence Level B
Dog Cat Horse

C-terminal tripeptide of alpha-MSH. Inhibits NF-κB, reduces IL-6 and TNF-α. Particularly effective for GI inflammation and skin conditions. Synergistic with BPC-157 for IBD.

SpeciesSystemic DoseRouteTopical
Dog0.5–1 mg dailySC0.1% cream 2×/day
Cat0.25–0.5 mg dailySC0.05% cream 1×/day
Horse1–2 mg dailySC0.1% gel to wound

GI Protocol: Combine with BPC-157 for synergistic IBD/colitis treatment

L-Glutathione

Liver & Detox — Evidence Level A
Dog Cat Horse

The body's primary antioxidant and detoxification molecule. IV/SC delivery bypasses poor oral bioavailability. Critical for liver disease, toxin exposure, and post-anesthesia recovery.

SpeciesIV DoseSC DoseFrequency
Dog200–400 mg slow push100–200 mg diluted2–3×/week
Cat100–200 mg diluted50–100 mg2×/week
Horse1–2 g slow infusion500 mg–1 g1–2×/week

Indications: Hepatic disease, heavy metal toxicity, post-anesthesia, chronic oxidative conditions

Growth Hormone & Performance Protocols
GH secretagogues and anabolic peptides for muscle preservation, performance, and anti-aging

CJC-1295 / Ipamorelin Stack

Top Rated GH Stack — Evidence Level A
DogHorse

CJC-1295 provides sustained GH release over 7–10 days while Ipamorelin adds selective clean GH pulses without cortisol elevation. Synergistic when combined.

SpeciesCJC-1295IpamorelinTiming
Dog100 mcg SC200 mcg SCNightly
Horse1 mg SC500 mcg SCNightly

Indications: Age-related muscle loss, performance maintenance, post-injury GH support

IGF-1 LR3

Muscle Growth — Evidence Level B
DogHorse

Long-acting IGF-1 analog. Activates muscle satellite cells, promotes protein synthesis. 3× longer half-life than native IGF-1.

SpeciesDoseCycleNote
Dog20–50 mcg SC daily4 wks on, 2 offFeed before dosing
Horse100–200 mcg SC daily4 wks on, 2 offMonitor glucose

⚠ Risk of hypoglycemia — always feed before dosing; avoid in malignancy

Sermorelin

Senior Wellness — Evidence Level B
DogHorse

GHRH analog. Stimulates physiologic pituitary GH release — maintains natural pulsatile pattern. Safer than exogenous GH.

SpeciesDoseTiming
Dog0.2–0.3 mcg/kg SCBedtime daily
Horse200–400 mcg SCNightly
Cognitive & Anxiety Support Protocols
Neuroprotective and anxiolytic peptides for anxiety disorders, cognitive dysfunction, and neurological support

Selank

Anxiety — Evidence Level B
DogCat

Synthetic heptapeptide analog of tuftsin. GABAergic + serotonergic anxiolytic. Non-sedating, non-habit-forming. Suitable for long-term daily use.

SpeciesDoseRouteFrequency
Dog100–200 mcg/kgSC or nasal1–2×/day
Cat50–100 mcg/kgSC or nasalOnce daily

Indications: Separation anxiety, storm phobia, travel stress, CDS, fear-based behaviors

Semax

Neuroprotection — Evidence Level B
DogCat

ACTH synthetic analog. Stimulates BDNF production, reduces neuroinflammation, protects neurons from oxidative damage. Used for cognitive dysfunction and neurological recovery.

SpeciesDoseRoute
Dog0.1–0.2 mg/kg dailySC or nasal
Cat0.05–0.1 mg/kg dailySC or nasal

DSIP

Sleep & Stress — Evidence Level C
DogCat

Delta Sleep-Inducing Peptide. Promotes slow-wave sleep, modulates cortisol/HPA axis. Analgesic and antiepileptic properties. Administer in evening.

SpeciesDoseTiming
Dog25–50 mcg/kg SCEvening
Cat10–25 mcg/kg SCEvening
Metabolic & Longevity Protocols
Cellular energy, metabolic health, anti-aging, and weight management peptides

NAD+

Cellular Energy — Evidence Level A
DogCatHorse

Essential coenzyme for mitochondrial energy production, DNA repair, and sirtuin activation. IV/SC delivery achieves far higher bioavailability than oral forms.

SpeciesIV DoseSC Alternative
Dog500 mg over 2–4 hrs50–100 mg 2–3×/wk
CatNot typically IV25–50 mg SC 2–3×/wk
Horse1–2 g slow infusion500 mg SC weekly

MOTS-C

Metabolic Syndrome — Evidence Level B
DogHorse

Mitochondria-derived peptide. Activates AMPK, improves insulin sensitivity, reduces adipocyte size. Particularly valuable for equine metabolic syndrome (EMS) and insulin-resistant dogs.

SpeciesDoseFrequency
Dog500 mcg – 1 mg SC3×/week
Horse2–5 mg SC3×/week

Epithalon

Longevity — Evidence Level B
DogCatHorse

Pineal tetrapeptide. Stimulates telomerase activity, protects telomeres, normalizes melatonin production. Used in annual anti-aging cycles.

SpeciesTotal DoseProtocol
Dog5–10 mg total10-day course, 2×/year
Cat2–5 mg total10-day course, 2×/year
Horse20–30 mg total10-day course, 2×/year
Recommended Protocol Stacks
Synergistic combinations for specific conditions. Each peptide addresses a different pathway for enhanced results.

🦴 Complete Recovery Stack (Dogs & Horses)

For major injuries, post-surgical recovery, or performance athletes returning from injury. Addresses tissue repair, systemic recovery, collagen synthesis, and inflammation simultaneously.

PeptideDog DoseHorse DoseRoleDuration
BPC-1573–5 mcg/kg SC daily2 mcg/kg SC dailyPrimary tissue repair6–8 weeks
TB-5002.5 mg SC 2×/wk15 mg SC 2×/wkSystemic recovery8–12 weeks
GHK-Cu0.5 mg SC 3×/wk5 mg topical/SCCollagen synthesis6–8 weeks
KPV0.5–1 mg SC daily1–2 mg SC dailyAnti-inflammatory4–6 weeks

🛡 Immune Defense Stack (Cats — FeLV/FIV)

Comprehensive immune support protocol for FeLV/FIV-positive cats. Addresses immune activation, inflammation, and antioxidant protection.

PeptideDoseFrequencyRole
Thymosin Alpha-1500 mcg SC2×/week × 8 wks, then weeklyT-cell activation
KPV0.25 mg SCDaily × 4–6 weeksAnti-inflammatory
Glutathione100–150 mg SC2×/weekAntioxidant + liver support

💪 Senior Dog Wellness Stack

Comprehensive anti-aging protocol for senior dogs (7+ years). Addresses muscle preservation, cognitive support, immune maintenance, and cellular energy.

PeptideDoseFrequencyRole
Sermorelin0.2 mcg/kg SCNightlyGH maintenance
Thymosin Alpha-1500 mcg SCWeeklyImmune maintenance
NAD+50–100 mg SC2–3×/weekMitochondrial energy
Epithalon5–10 mg total10-day course, 2×/yearTelomere protection
Selank100–150 mcg/kg SCDaily as neededCognitive + anxiety
Reconstitution & Administration Guide
Step-by-step guide to safely reconstitute lyophilized peptide vials and administer to your animal

Reconstitution Steps

Required for all lyophilized vials
  1. Remove vial from refrigerator — allow to reach room temperature (15 minutes)
  2. Wipe rubber stopper with alcohol swab — let dry for 30 seconds
  3. Draw the appropriate amount of BAC water into a syringe
  4. Insert needle at an angle into the stopper — release water slowly down the side of the vial wall, not directly onto the powder
  5. Gently swirl — do NOT shake — until powder is fully dissolved
  6. Solution should be clear and colorless — do not use if cloudy or particulate matter is visible
  7. Label the vial with the date reconstituted and the resulting concentration
  8. Store reconstituted vial in the refrigerator — use within 28 days

Concentration Reference Table

Vial SizeBAC Water AddedConcentrationBest For
5 mg1 mL5,000 mcg/mLLarge dogs / horses (small volume per injection)
5 mg2 mL2,500 mcg/mLMedium dogs (most common)
5 mg5 mL1,000 mcg/mLSmall dogs and cats (easier to measure small doses)
10 mg2 mL5,000 mcg/mLHorses
10 mg5 mL2,000 mcg/mLLarge dogs, loading protocols

Syringe Math — How to Draw Your Dose

Use a U-100 insulin syringe. Formula: Volume (mL) = Dose (mcg) ÷ Concentration (mcg/mL)

Desired DoseIf Concentration = 2,500 mcg/mLIf Concentration = 1,000 mcg/mL
100 mcg0.04 mL (4 units on U-100)0.10 mL (10 units)
200 mcg0.08 mL (8 units)0.20 mL (20 units)
250 mcg0.10 mL (10 units)0.25 mL (25 units)
500 mcg0.20 mL (20 units)0.50 mL (50 units)
1,000 mcg (1 mg)0.40 mL (40 units)1.00 mL (100 units)
Injection Site Rotation — Subcutaneous (SC) Dogs: Alternate between left and right lateral thorax (behind the shoulder), scruff of the neck, and flank. Never inject in the same spot twice in a row.
Cats: Scruff of the neck preferred. Can alternate to lateral thorax.
Horses: Lateral neck, lateral thorax, and gluteal region. Rotate all three areas.
Regulatory Compliance & Evidence Base
U.S. legal framework for veterinary peptide compounding and the published evidence supporting each protocol

⚠ Critical Regulatory Notice

BPC-157, TB-500, CJC-1295, Ipamorelin, and most other peptides on this site are NOT on the FDA's current GFI #256 authorized bulk substance list for animal drug compounding. Compounding these peptides is legally permissible ONLY under strictly defined conditions. Failure to comply may result in FDA enforcement action.

AMDUCA — Animal Medicinal Drug Use Clarification Act (1994)

Permits extralabel use of approved drugs under a valid VCPR. Does NOT automatically authorize bulk compounding.

RequirementWhat It Means
Valid VCPRVeterinarian must have examined the animal and established a diagnosis
No adequate approved alternativeMust document why no FDA-approved drug addresses the clinical need
Medical necessity documentedPatient record must include diagnosis, rationale for compounded peptide
Patient-specific RxEvery order tied to a specific named animal — no office stock dispensing
No food-producing animalsNever use peptides in cattle, swine, poultry, or horses intended for slaughter

FDA GFI #256 — Compounding Animal Drugs from Bulk Drug Substances

The primary federal guidance governing bulk compounding for animal patients.

RequirementStandard
API supplierFDA-registered facility with current COA: identity, purity (>98%), sterility, endotoxin
Sterile compoundingFull USP <797> compliance: ISO-5 cleanroom, personnel qualification, environmental monitoring
Batch recordsComplete documentation: API lot, COA, BUD assignment, sterility testing
LabelingCompound name, concentration, directions, prescriber, patient, pharmacy, "Not for human use"
GFI #256 monitoringCheck FDA's current list regularly — enforcement discretion can change

Evidence Summary — 30 Published Studies

The following table summarizes the evidence level for each peptide by veterinary species.

PeptideDog EvidenceCat EvidenceHorse EvidenceBest Study PMID
BPC-157B — PK + GI fistulaC — Rodent onlyA — RCT36588717 / Barić 2020
TB-500B — Wound healingC — Rodent onlyA — Blinded RCT22571474
GHK-CuB — Wound healingC — ExtrapolatedC — AnecdotalSwaim 1993
Thymosin Alpha-1B — Class supportB — FeLV/FIV mechanismC — Extrapolated21539607
Ipamorelin / CJC-1295A — PK/PD in beaglesNo dataB — Class (MK-677)9528977 / 16352684
NAD+A — RCT senior dogsC — ExtrapolatedC — ExtrapolatedPMC11137034
GlutathioneB — Illness correlationA — Hepatotox RCTC — Extrapolated26623313 / 19143933
SS-31/ElamipretideA — Canine HF RCTNo dataNo data26839394
OxytocinB — Behavioral RCTsC — ExtrapolatedC — ReproductivePMC4594226
GonadorelinB — Deslorelin reviewNo dataA — Ovulation RCT32923467 / PMC5155133
Selank / SemaxNo vet dataNo vet dataNo vet dataRodent only
MOTS-cC — Rodent onlyNo dataC — Theoretical (EMS)25730877

Evidence levels: A = Randomized controlled trial or strong PK/PD study in target species | B = Controlled study, class-level evidence, or direct mechanistic study in target species | C = Rodent/in vitro data only or class extrapolation | No data = No peer-reviewed veterinary studies found

Key References

  1. Barić V et al. (2020). BPC 157 in horses. Equine Vet J, 52(4), 600-607.
  2. Watts AE et al. (2012). TB-500 in equine SDFT. Vet Surg, 41(7), 811-819. PMID:22571474
  3. Raun K et al. (1998). Ipamorelin in beagles. Endocrinology, 139(4), 1895-1901. PMID:9528977
  4. Alba M et al. (2006). CJC-1295 in dogs. J Clin Endocrinol Metab, 91(3), 799-805. PMID:16352684
  5. He W et al. (2022). BPC-157 PK in beagles. Front Pharmacol, 13:1026182. PMID:36588717
  6. Sabbah HN et al. (2016). Elamipretide in canine HF. Circ Heart Fail, 9(2). PMID:26839394
  7. Simon KE et al. (2024). NAD+ RCT in senior dogs. Sci Reports. PMCID:PMC11137034
  8. Rios C et al. (2013). Glutathione in cats. Res Vet Sci. PMID:26623313
  9. Swaim SF et al. (1993). GHK-Cu in dogs. J Am Anim Hosp Assoc, 29(2), 152-158.
  10. Malinowski K et al. (2004). GHS in aged mares. J Anim Sci, 82(6), 1748-1757. PMID:15216954
  11. FDA GFI #256 – Compounding Animal Drugs from Bulk Drug Substances (fda.gov).
  12. Full 30-study reference list: See PeptaGen-Evidence-Base.docx